Home
Resources
Publications
Application of N-Dodecane as an Oxygen Vector to Enhance the Activity of Fumarase in Recombinant Escherichia Coli: Role of Intracellular Microenvironment

Application of N-Dodecane as an Oxygen Vector to Enhance the Activity of Fumarase in Recombinant Escherichia Coli: Role of Intracellular Microenvironment

Sen Zhang, Ping Song, Shuang Li

Braz J Microbiol. Jul-Sep 2018;49(3):662-667.

PMID: 29409732

Abstract:

The effect of the intracellular microenvironment in the presence of an oxygen vector during expression of a fusion protein in Escherichia coli was studied. Three organic solutions at different concentration were chosen as oxygen vectors for fumarase expression. The addition of n-dodecane did not induce a significant change in the expression of fumarase, while the activity of fumarase increased significantly to 124% at 2.5% n-dodecane added after 9h induction. The concentration of ATP increased sharply during the first 6h of induction, to a value 7600% higher than that in the absence of an oxygen-vector. NAD/NADH and NADP/NADPH ratios were positively correlated with fumarase activity. n-Dodecane can be used to increase the concentration of ATP and change the energy metabolic pathway, providing sufficient energy for fumarase folding.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP112403	Dodecane		112-40-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: