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Arachidonic Acid Formed by Peroxisomal Beta-Oxidation of 7,10,13,16-docosatetraenoic Acid Is Esterified Into 1-acyl-sn-glycero-3-phosphocholine by Microsomes

Arachidonic Acid Formed by Peroxisomal Beta-Oxidation of 7,10,13,16-docosatetraenoic Acid Is Esterified Into 1-acyl-sn-glycero-3-phosphocholine by Microsomes

S P Baykousheva, D L Luthria, H Sprecher

J Biol Chem. 1994 Jul 15;269(28):18390-4.

PMID: 8034586

Abstract:

Peroxisomal beta-oxidation of linoleic acid and arachidonic acid was depressed when 1-palmitoyl-sn-glycero-3-phosphocholine and microsomes were included in incubations. This reduction was due to the esterification of the substrate into the acceptor by microsomal 1-acyl-sn-glycero-3- phosphocholine acyltransferase. The first cycle of the beta-oxidation of 7,10,13,16-docosatetraenoic acid was independent of 1-acyl-sn-glycero-3-phosphocholine and microsomes. However, when arachidonate was produced it was esterified rather than serving as a substrate for continued beta-oxidation. When arachidonate and linoleate were incubated with peroxisomes alone, 2-trans-4,7,10-hexadecatetraenoic acid and 2-trans-4-decadienoic acid were the respective end products of beta-oxidation. 2-Oxo-8,11-heptadecadienone, a catabolite produced from linoleate, was most likely a nonenzymatic decarboxylation product of 3-oxo-9,12-octadecadienoic acid. In addition to the termination of beta-oxidation by microsomal-peroxisomal communication, our results with linoleate and arachidonate suggest that the reaction catalyzed by 2-trans-4-cis-dienoyl-CoA reductase is the control step in double bond removal. In addition, the beta-ketothiolase step may play a regulatory role in the peroxisomal beta-oxidation of linoleate but not arachidonate or 7,10,13,16-docosatetraenoic acid.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP17364168	1-Palmitoyl-sn-glycero-3-phosphocholine		17364-16-8	Price

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