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Ascorbyl palmitate/DSPE-PEG Nanocarriers for Oral Iron Delivery: Preparation, Characterisation and in Vitro Evaluation

Ascorbyl palmitate/DSPE-PEG Nanocarriers for Oral Iron Delivery: Preparation, Characterisation and in Vitro Evaluation

M Gulrez Zariwala, Sebastien Farnaud, Zahra Merchant, Satyanarayana Somavarapu, Derek Renshaw

Colloids Surf B Biointerfaces. 2014 Mar 1;115:86-92.

PMID: 24333557

Abstract:

The objective of this study was to encapsulate iron in nanocarriers formulated with ascorbyl palmitate and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine polyethylene glycol (DSPE-PEG) for oral delivery. Blank and iron (Fe) loaded nanocarriers were prepared by a modified thin film method using ascorbyl palmitate and DSPE-PEG. Surface charge of the nanocarriers was modified by the inclusion of chitosan (CHI) during the formulation process. Blank and iron loaded ascorbyl palmitate/DSPE nanocarriers were visualised by transmission electron microscopy (TEM) and physiochemical characterisations of the nanocarriers carried out to determine the mean particle size and zeta potential. Inclusion of chitosan imparted a net positive charge on the nanocarrier surface and also led to an increase in mean particle size. Iron entrapment in ascorbyl palmitate-Fe and ascorbyl palmitate-CHI-Fe nanocarriers was 67% and 76% respectively, suggesting a beneficial effect of chitosan on nanocarrier Fe entrapment. Iron absorption was estimated by measuring Caco-2 cell ferritin formation using ferrous sulphate as a reference standard. Iron absorption from ascorbyl palmitate-Fe (592.17±21.12 ng/mg cell protein) and ascorbyl palmitate-CHI-Fe (800.12±47.6 ng/mg, cell protein) nanocarriers was 1.35-fold and 1.5-fold higher than that from free ferrous sulphate, respectively (505.74±23.73 ng/mg cell protein) (n=6, p<0.05). This study demonstrates for the first time preparation and characterisation of iron loaded ascorbyl palmitate/DSPE PEG nanocarriers, and that engineering of the nanocarriers with chitosan leads to a significant augmentation of iron absorption.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP137666-A	Ascorbyl Palmitate		137-66-6	Price

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