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Assay of Plasma Leupeptin Using the Reversible Binding of Leupeptin to Bovine Pancreatic Trypsin

Assay of Plasma Leupeptin Using the Reversible Binding of Leupeptin to Bovine Pancreatic Trypsin

K Yamashita, K Watanabe, H Takayama, S Mizuguchi, M Ishibashi, H Miyazaki, W Tanaka, H Umezawa

Anal Biochem. 1986 Aug 1;156(2):503-7.

PMID: 3766949

Abstract:

A competitive binding radioassay for leupeptin has been developed utilizing the reversible binding of leupeptin to bovine pancreatic trypsin. An ethanol precipitation step was introduced to separate trypsin-bound leupeptin from its free form. Advantages of this method are simplicity of the procedure and avoidance of the preparation of antiserum. The possible metabolites of leupeptin exhibit no significant inhibitory effect on leupeptin-trypsin binding in this system. This method was applied to the determination of plasma leupeptin levels in dogs after oral administration of the peptide.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP103476897	Leupeptin		103476-89-7	Price

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