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Assembly of Multilayer PSS/PAH Membrane on Coherent alginate/PLO Microcapsule for Long-Term Graft Transplantation

Assembly of Multilayer PSS/PAH Membrane on Coherent alginate/PLO Microcapsule for Long-Term Graft Transplantation

Andy Leung, Matt Trau, Lars Keld Nielsen

J Biomed Mater Res A. 2009 Jan;88(1):226-37.

PMID: 18286625

Abstract:

Conventional alginate/poly-L-ornithine (AP) membranes used to immunoisolate foreign tissue transplants fail in long-term transplantations of immortal cell lines. We have developed a novel layer-by-layer (LbL) membrane using polystyrene sulfonate and polyallylamine hydrochloride (PSS/PAH) on top of the coherent AP membrane. Assembly of the LbL membrane was followed by electrophoresis, and the surface morphologies and structure were characterized and examined by cryo-scanning electron microscope and transmission electron microscopy. Unlike the standard AP membrane, the LbL membrane withstood the internal pressure generated by continuous cell proliferation of microencapsulated HEK-293 and Min-6 cells. The new membrane did not affect insulin secretion or diffusion by Min-6 cells.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP26982218	Poly-L-ornithine hydrochloride		26982-21-8	Price

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