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[Assessment of the Possibility of Application in Laboratory Practice of Reagent Kit for Diagnosis of Glanders and Melioidosis by Real-Time Polymerase Chain Reaction]

[Assessment of the Possibility of Application in Laboratory Practice of Reagent Kit for Diagnosis of Glanders and Melioidosis by Real-Time Polymerase Chain Reaction]

L V Lemasova, G A Tkachenko, E V Prokhvatilova, L I Belitskaya, D V Viktorov, A V Toporkov

Klin Lab Diagn. 2019;64(11):700-704.

PMID: 31747502

Abstract:

The reagent kit AmpligenBurk-mallei/pseudomallei-RT PCR is designed for detecting in vitro diagnostics and differentiate the DNA of glanders and melioidosis pathogens by real-time multiplex PCR in biological (clinical) material and cultures of microorganisms, as well as environmental objects and solid food products (rice). During clinical testing diagnostic value of reagent kit AmpligenBurk-mallei/pseudomallei-RT PCR has been studied. Based on the results obtained, a high analytical sensitivity (1×103 microbe cells/ml) and specificity (100%) of PCR-RT with the developed reagent kit were established, regardless of the type of material being studied. The diagnostic sensitivity of PCR-RT using a set of reagents was at least 98.0% and specificity at least 99%. The stages of state examination have been completed, a registration certificate has been obtained at Roszdravnadzor, production, sale and use of reagent kit in medical laboratory practice have been permitted.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP8007098	Eschka's Reagent		8007-09-8	Price

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