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Asymmetric Synthesis of (S)-alpha-methylbenzylamine by Recombinant Escherichia Coli Co-Expressing Omega-Transaminase and Acetolactate Synthase

Hyungdon Yun, Byung-Gee Kim

Biosci Biotechnol Biochem. 2008 Nov;72(11):3030-3.

PMID: 18997410

Abstract:

To produce (S)-alpha-methylbenzylamine (MBA) from acetophenone, recombinant Escherichia coli co-expressing omega-transaminase and acetolactate synthase was used as a whole-cell biocatalyst. The solvent-bridge reaction system increased the yield of the whole-cell reaction by 2.5-fold, and the inhibitory (S)-alpha-MBA produced in the omega-transaminase reaction solution (pH 8.0) moved into the extraction solution (pH 3.0) via an organic solvent.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP2627863 (S)-(−)-α-Methylbenzylamine (S)-(−)-α-Methylbenzylamine 2627-86-3 Price
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