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ATM Is the Predominant Kinase Involved in the Phosphorylation of Histone H2AX After Heating

ATM Is the Predominant Kinase Involved in the Phosphorylation of Histone H2AX After Heating

Akihisa Takahashi, Eiichiro Mori, Xiaoming Su, Yosuke Nakagawa, Noritomo Okamoto, Hirokazu Uemura, Natsuko Kondo, Taichi Noda, Atsushi Toki, Yosuke Ejima, David J Chen, Ken Ohnishi, Takeo Ohnishi

J Radiat Res. 2010;51(4):417-22.

PMID: 20448412

Abstract:

Heating induces histone H2AX phosphorylation at serine 139 (gammaH2AX). Phosphorylated H2AX subsequently forms foci in numerous mammalian cell lines. The aim of this study was to clarify details in the mechanisms involved in the phosphorylation of H2AX after heating. The cell lines used were DNA-PKcs knockout cells, ATM knockout cells, and their parental cell lines. To elucidate mechanisms of induction of phosphorylation of H2AX after heating, ATM/ATR inhibitor (CGK733) and DNA-PK inhibitor (NU7026) were used. The intensity of gammaH2AX signals was assayed with flow cytometry. The thermal dose-response curve for the fluorescence intensity of gammaH2AX appearance in DNA-PKcs-/- cells during the heating period was similar to that observed in DNA-PKcs+/+ cells. On the other hand, the slope of thermal dose-response curve for them in ATM-/- cells was lower than that in ATM+/+ cells. Phosphorylation of H2AX after heating was suppressed by a combination of CGK733 and NU7026 in the culture medium in DNA-PKcs-/- cells, ATM-/- cells and in their parental cells. Although the phosphorylation of H2AX after heating was not suppressed by NU7026 in their parental cells, such phosphorylation was suppressed by CGK733 in their parental cells. These results indicate that ATM is the predominant protein which is active in the phosphorylation of histone H2AX after heating.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4242694	CGK733			Price

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