Home
Resources
Publications
Atomic Absorption Spectrometric Detection of Biotin-Cymantrene as a Metallo-Tracer for the Avidin-Biotin System

Atomic Absorption Spectrometric Detection of Biotin-Cymantrene as a Metallo-Tracer for the Avidin-Biotin System

I Rémy, P Brossier

Analyst. 1993 Aug;118(8):1021-5.

PMID: 8214606

Abstract:

Biotin-cyclopentadienylmanganese tricarbonyl (biotin-cymantrene; biotin-Cy) is proposed as a universal metallo-tracer for immunoassays. Associated with the possibility of detecting this type of label (organometallic moieties) along with different analytical procedures, this reagent appears to be a universal system with the advantages of being inexpensive, stable and directly detected (no substrate is required). The optimum conditions for detecting the organometallic label biotin-Cy with a Zeeman atomic absorption spectrometer and the affinity of biotin-Cy for the streptavidin ligand immobilized on the walls of microtitre plates, wells or tubes are described. The results obtained demonstrate the possibility of assaying a wide range of clinical parameters because the detection limit of biotin-Cy is about 1 pg per assay. Based on the competition between immobilized bovine serum albumin adsorbed on the wall and free bovine serum albumin for specific polyclonal antibodies, the streptavidin-metallo-biotin system was tested on a competitive immunoassay model and a satisfactory calibration graph was obtained.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP12079651	Cyclopentadienylmanganese(I) tricarbonyl		12079-65-1	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: