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Attenuated Airway Eosinophilic Inflammations in IL-38 Knockout Mouse Model

Attenuated Airway Eosinophilic Inflammations in IL-38 Knockout Mouse Model

Masanobu Matsuoka, Tomotaka Kawayama, Masaki Tominaga, Shinjiro Kaieda, Yoshihisa Tokunaga, Yoichiro Kaku, Haruki Imaoka, Takashi Kinoshita, Masaki Okamoto, Jun Akiba, Tomoaki Hoshino

Kurume Med J. 2019 May 16;65(2):37-46.

PMID: 30853691

Abstract:

Background:
The role of IL-38, a new member of the IL-1 family, in airway eosinophilic inflammatory conditions such as asthma is unclear. To investigate the role of IL-38 in airway eosinophilic inflammation, an IL-38-gene deficient (KO) murine asthma model was analyzed.
Methods:
The numbers of eosinophils and neutrophils, and levels of IL-5, IL-13 and IL-17A protein and mRNA in bronchoalveolar lavage fluid (BALF) and lung tissue were compared between wild-type (WT) and IL-38-KO mice after OVA sensitization and challenge. The effects of additional purified recombinant mouse (rm) IL-38 protein were investigated in the IL-38-KO murine asthma model.
Results:
The IL-38 and IL-5 mRNA in WT mice was significantly higher after OVA challenge than after saline challenge (p<0.05). The number of airway eosinophils in IL-38-KO mice was significantly lower than in WT mice after OVA challenge (p<0.01). BALF analysis confirmed the lower number of airway eosinophils in IL-38-KO mice and showed that this was significantly associated with lower IL-5 protein levels (r=0.92, p<0.0001). However, the additional rm IL-38 protein did not neutralize airway eosinophilia in IL-38-KO mice.
Conclusion:
IL-38 may enhance airway eosinophilic inflammation in asthma through IL-5 induction.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413273	IL-5 from mouse			Price

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