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Autocrine IL-10 Promotes Human B-cell Differentiation Into IgM- Or IgG-secreting Plasmablasts

Autocrine IL-10 Promotes Human B-cell Differentiation Into IgM- Or IgG-secreting Plasmablasts

Guido Heine, Gennadiy Drozdenko, Joachim R Grün, Hyun-Dong Chang, Andreas Radbruch, Margitta Worm

Eur J Immunol. 2014 Jun;44(6):1615-21.

PMID: 24643722

Abstract:

B-cell-derived interleukin-10 (IL-10) is known to act in a paracrine fashion to suppress inflammation. Here, we show that IL-10 also acts in an autocrine manner to regulate the differentiation of activated human B cells. We report that IL-10 expression is not restricted to a dedicated B-cell subset, but is induced transiently in peripheral human naïve, memory, and CD5(+) B cells alike upon activation. Global transcriptome comparison of activated human B cells, secreting IL-10 or not, identified 138 differentially regulated genes, most of which were associated with differentiation into antibody-secreting cells and reflecting autocrine IL-10 signaling. We monitored the differentiation of IL-10-secreting B cells and determined the effect of IL-10-blocking antibodies against its autocrine and paracrine signaling. IL-10 signaling promoted the differentiation of activated IL-10-secreting B cells into IgM- or IgG-secreting cells, but was dispensable for IgA secretion. Our data imply that B-cell-derived IL-10 not only suppresses immune reactions via paracrine mechanisms, but can also contribute to the differentiation of IL-10-secreting B cells into IgM- and IgG-secreting plasmablasts through both autocrine and paracrine signaling.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248648	IL-10 human			Price

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