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Autocrine/paracrine Induction of Tissue Inhibitor of metalloproteinase-1 in Chinese Hamster Ovary Cells by Oncostatin M

Autocrine/paracrine Induction of Tissue Inhibitor of metalloproteinase-1 in Chinese Hamster Ovary Cells by Oncostatin M

N Malik, B Evans, B W Greenfield, R A Shapiro, M Hanson, M Shoyab

Matrix Biol. 1995 Oct;14(8):677-80.

PMID: 9057817

Abstract:

Chinese hamster ovary (CHO) cells expressing recombinant human oncostatin M (rOM) were found to secrete high levels of a 28-kDa protein. Sequence analysis of the protein suggested that it was hamster tissue inhibitor of metalloproteinase-1 (TIMP-1). In this study, we show that induction of TIMP-1 mRNA and protein by CHO cells is due to rOM action in an autocrine/paracrine mode. TIMP-1 expression in rOM-producing CHO cells increased concomitantly with methotrexate-induced rOM amplification. TIMP-1 upregulation was not caused by either transfection of nonspecific DNA nor was it a direct effect of treatment of the cells with methotrexate. These results suggest that oncostatin M is a potent inducer of TIMP-1 and that its receptor-mediated expression is conserved across species.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4241305	TIMP-1, Human, Recombinant, CHO Cells			Price

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