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BDNF-Live-Exon-Visualization (BLEV) Allows Differential Detection of BDNF Transcripts

BDNF-Live-Exon-Visualization (BLEV) Allows Differential Detection of BDNF Transcripts

Wibke Singer, Marie Manthey, Rama Panford-Walsh, Lucas Matt, Hyun-Soon Geisler, Eleonora Passeri, Gabriele Baj, Enrico Tongiorgi, Graciano Leal, Carlos B Duarte, Ivan L Salazar, Philipp Eckert, Karin Rohbock, etc.

Front Mol Neurosci. 2018 Sep 27;11:325.

PMID: 30319348

Abstract:

Bdnf exon-IV and exon-VI transcripts are driven by neuronal activity and are involved in pathologies related to sleep, fear or memory disorders. However, how their differential transcription translates activity changes into long-lasting network changes is elusive. Aiming to trace specifically the network controlled by exon-IV and -VI derived BDNF during activity-dependent plasticity changes, we generated a transgenic reporter mouse for B DNF- l ive- e xon- v isualization (BLEV), in which expression of Bdnf exon-IV and -VI can be visualized by co-expression of CFP and YFP. CFP and YFP expression was differentially activated and targeted in cell lines, primary cultures and BLEV reporter mice without interfering with BDNF protein synthesis. CFP and YFP expression, moreover, overlapped with BDNF protein expression in defined hippocampal neuronal, glial and vascular locations in vivo. So far, activity-dependent BDNF cannot be explicitly monitored independent of basal BDNF levels. The BLEV reporter mouse therefore provides a new model, which can be used to test whether stimulus-induced activity-dependent changes in BDNF expression are instrumental for long-lasting plasticity modifications.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42412798	Neuronal Differentiation Inducer IV			Price

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