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Best Practices and Benchmarks for Intact Protein Analysis for Top-Down Mass Spectrometry

Best Practices and Benchmarks for Intact Protein Analysis for Top-Down Mass Spectrometry

Daniel P Donnelly, Catherine M Rawlins, Caroline J DeHart, Luca Fornelli, Luis F Schachner, Ziqing Lin, Jennifer L Lippens, Krishna C Aluri, Richa Sarin, Bifan Chen, Carter Lantz, Wonhyeuk Jung, Kendall R Johnson, etc.

Nat Methods. 2019 Jul;16(7):587-594.

PMID: 31249407

Abstract:

One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9048468-B	Protein standard		9048-46-8	Price

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