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Beta-Galactosidase Assay Using Capillary Electrophoresis Laser-Induced Fluorescence Detection and resorufin-beta-D-galactopyranoside as Substrate

M J Eggertson, D B Craig

Biomed Chromatogr. 1999 Dec;13(8):516-9.

PMID: 10611604

Abstract:

beta-Galactosidase was incubated for 60 min with the fluorogenic substrate resorufin-beta-D-galactopyranoside, which is converted by the action of the enzyme into resorufin and galactose. A 160 pL aliquot of reaction mixture was analyzed by capillary electrophoresis utilizing laser-induced fluorescence detection. Based on the detection of the resorufin formed, the limit of detection of beta-galactosidase was 1.5 x 10(-15) M or 900 molecules of enzyme in a 1 microL sample.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP95079199 Resorufin β-D-galactopyranoside Resorufin β-D-galactopyranoside 95079-19-9 Price
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