Home
Resources
Publications
Binding of an Inert, Cationic Osmium Probe to Walls of Bacillus Subtilis

Binding of an Inert, Cationic Osmium Probe to Walls of Bacillus Subtilis

T J Beveridge, T Jack

J Bacteriol. 1982 Mar;149(3):1120-3.

PMID: 6801010

Abstract:

X-ray fluorescence spectroscopy and electron microscopy of unstained specimens have been used to study the binding of chloropentaammineosmium(III) chloride to isolated walls of Bacillus subtilis. Native walls bound 0.220 mumol of the osmium probe per mg (dry weight) of walls, whereas walls which were chemically treated to neutralize the available carboxylate groups of the peptidoglycan bound only 0.040 mumol. Teichoic acid-depleted walls bound 0.210 mumol. Thin sections of all wall types showed the osmium probe to be scattered throughout the wall matrix as a small staining deposit. The results support the idea that the metal ion-binding capacity of these walls is mediated by the available carboxylate groups in the wall fabric.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP13444934	Osmium(III) chloride		13444-93-4	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: