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Binding of Iron and Inhibition of Iron-Dependent Oxidative Cell Injury by the "Calcium Chelator" 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic Acid (BAPTA)

Binding of Iron and Inhibition of Iron-Dependent Oxidative Cell Injury by the "Calcium Chelator" 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic Acid (BAPTA)

B E Britigan, G T Rasmussen, C D Cox

Biochem Pharmacol. 1998 Feb 1;55(3):287-95.

PMID: 9484794

Abstract:

A role for increases in intracellular calcium (Ca2+) has been suggested in the pathophysiology of various forms of oxidant-mediated cell injury. In recent studies, we found that iron bound to the Pseudomonas aeruginosa siderophore, pyochelin, augments oxidant-mediated endothelial cell injury by catalyzing the formation of hydroxyl radical (HO.). To investigate the role of Ca2+ in this process, the effects of two Ca2+ chelating agents, Fura-2 and 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid (BAPTA), were assessed. BAPTA, but not Fura-2, was protective against H2O2/ferripyochelin-mediated injury. Subsequent data suggested that chelation of iron rather than Ca2+ by BAPTA was most likely responsible. Spectrophotometry demonstrated that both ferrous (Fe2+) and ferric (Fe3+) iron formed a complex with BAPTA. The affinity of BAPTA for the metals was Fe3+ > Ca2+ > Fe2+. BAPTA was found to decrease markedly iron-catalyzed production of HO. and/or ferryl species when analyzed by spin trapping. Although our results do not definitively prove that BAPTA protects endothelial cells from ferripyochelin-associated damage by chelating iron, these data indicate that caution must be exercised in utilizing protective effects of intracellular "Ca2+ chelating agents" as evidence for a role of alterations in cellular Ca2+ levels in experimental conditions in which iron-mediated oxidant production is also occurring.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP85233198	1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid		85233-19-8	Price

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