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Biocatalytic Action of Proteases in Ionic Liquids: Improvements on Their Enzymatic Activity, Thermal Stability and Kinetic Parameters

Biocatalytic Action of Proteases in Ionic Liquids: Improvements on Their Enzymatic Activity, Thermal Stability and Kinetic Parameters

Vítor Geniselli da Silva, Ruann Janser Soares de Castro

Int J Biol Macromol. 2018 Jul 15;114:124-129.

PMID: 29567497

Abstract:

This study evaluated the effect of the addition of the following ionic liquids (IL): choline chloride (CC), tetramethylammonium bromide (TB) and 1‑ethyl‑3‑methylimidazolium bromide (EM), on some biochemical properties including enzymatic activity and different kinetic parameters of commercial proteases. The enzyme-IL combinations that showed the highest increases in enzyme activities were as follows: CC (0.5mM) and Neutrase® 0.8L; CC (5mM) and Flavourzyme® 500L; TB (2000mM) and Alcalase® 2.4L, with relative increases of 20, 15 and 150% in protease activities, respectively, compared to the control assays. The combination TB and Alcalase® 2.4L showed a reduction of 50% of the activation energy (Ea), an increase of the relation Vmax/Km of 35% and a 16-fold rise in the values of t1/2, and D. Neutrase® 0.8L combined with CC showed an increase of 20% in the relation Vmax/Km. The combination Flavourzyme® 500L and CC presented a 20% higher value of the relation Vmax/Km and a 2-fold increase in the values of t1/2 and D compared to the control assay. In summary, the most positive effects observed in this study included proteases with improved activity and stability properties and a greater affinity for the substrate.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP64200	Tetramethylammonium bromide		64-20-0	Price

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