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Bioconjugation of Antibodies to Horseradish Peroxidase (HRP)

Robert M Hnasko

Methods Mol Biol. 2015;1318:43-50.

PMID: 26160562

Abstract:

The bioconjugation of an antibody to an enzymatic reporter such as horseradish peroxidase (HRP) affords an effective mechanism by which immunoassay detection of a target antigen can be achieved. The use of heterobifunctional cross-linkers to covalently link antibodies to HRP provides a simple and convenient means to maintain antibody affinity while imparting a functional reporter used for antigen detection. In this chapter, we describe a process by which Sulfo-SMCC is used to generate a stable maleimide-activated HRP that is reactive with sulfhydryl groups generated in antibodies by SATA-mediated thiolation.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR424824 Peroxidase, Maleimide Activated from horseradish Peroxidase, Maleimide Activated from horseradish Price
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