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Biodegradation of fenoxaprop-P-ethyl (FE) by Acinetobacter Sp. Strain DL-2 and Cloning of FE Hydrolase Gene afeH

Biodegradation of fenoxaprop-P-ethyl (FE) by Acinetobacter Sp. Strain DL-2 and Cloning of FE Hydrolase Gene afeH

Weiliang Dong, Sheng Jiang, Kaiwen Shi, Fei Wang, Shuhuan Li, Jie Zhou, Fei Huang, Yicheng Wang, Yuxiao Zheng, Ying Hou, Yan Huang, Zhongli Cui

Bioresour Technol. 2015 Jun;186:114-121.

PMID: 25812814

Abstract:

Fenoxaprop-P-ethyl (FE) is widely used as a post-emergence aryloxyphenoxy propionate (AOPP) herbicide in agriculture. An efficient FE-degrading strain DL-2 was isolated from the enrichment culture and identified as Acinetobacter sp. and the metabolite fenoxaprop acid (FA) was identified by HPLC/MS analysis. The strain DL-2 could also degrade a wide range of other AOPP herbicides. A novel FE hydrolase esterase gene afeH was cloned from strain DL-2 and functionally expressed in Escherichia coli BL21(DE3). The specific activities of recombinant AfeH was 216.39 U mg(-1) for FE with Km and Vmax values of 0.82 μM and 7.94 μmol min(-1) mg(-1). AfeH could also hydrolyze various AOPP herbicides, p-nitrophenyl esters and triglycerides. The optimal pH and temperature for recombinant AfeH were 9.0 and 50°C, respectively; the enzyme was activated by Co(2+) and inhibited by Ca(2+), Zn(2+), Ba(2+). AfeH was inhibited strongly by phenylmethylsulfonyl and SDS and weakly by dimethyl sulfoxide.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP71283802	Fenoxaprop-P-ethyl		71283-80-2	Price

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