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Biodiesel Production From Nannochloropsis Gaditana Lipids Through Transesterification Catalyzed by Rhizopus Oryzae Lipase

Biodiesel Production From Nannochloropsis Gaditana Lipids Through Transesterification Catalyzed by Rhizopus Oryzae Lipase

Elvira Navarro López, Alfonso Robles Medina, Pedro Antonio González Moreno, Luis Esteban Cerdán, Lorena Martín Valverde, Emilio Molina Grima

Bioresour Technol. 2016 Mar;203:236-44.

PMID: 26735878

Abstract:

Biodiesel (fatty acid methyl esters, FAMEs) was produced from saponifiable lipids (SLs) extracted from wet Nannochloropsis gaditana biomass using methanolysis catalyzed by Rhizopus oryzae intracellular lipase. SLs were firstly extracted with ethanol to obtain 31 wt% pure SLs. But this low SL purity also gave a low biodiesel conversion (58%). This conversion increased up to 80% using SLs purified by crystallization in acetone (95 wt% purity). Polar lipids play an important role in decreasing the reaction velocity - using SLs extracted with hexane, which have lower polar lipid content (37.4% versus 49.0% using ethanol), we obtained higher reaction velocities and less FAME conversion decrease when the same lipase batch was reused. 83% of SLs were transformed to biodiesel using a 70 wt% lipase/SL ratio, 11:1 methanol/SL molar ratio, 10 mL t-butanol/g SLs after 72 h. The FAME conversion decreased to 71% after catalyzing three reactions with the same lipase batch.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9016186-F	Esterase from Rhizopus oryzae		9016-18-6	Price

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