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Biological Effects of a Rat Monoclonal Anti-Mouse IFN-gamma Antibody Produced by in Vitro Immunization

Biological Effects of a Rat Monoclonal Anti-Mouse IFN-gamma Antibody Produced by in Vitro Immunization

B Echtenacher, R Hederer, P H Krammer

Immunobiology. 1987 Dec;176(1-2):96-107.

PMID: 3129363

Abstract:

This paper describes the biological effect of monoclonal antibodies to murine IFN-gamma produced by in vitro immunization with only several nanograms of rIFN-gamma. Four mAbs binding to rIFN-gamma were selected. mAb U7 was characterized in detail and shown to bind specifically to rIFN-gamma in a Western blot and to specifically inhibit the antiviral effect of rIFN-gamma and natural IFN-gamma. The activities of IFN-alpha, beta and IL2 were not affected. The M phi activating effect of both rIFN-gamma and natural IFN-gamma was also inhibited by mAb U7. Thus, we showed that it is possible to produce specific mAbs with very small amounts of cytokines by in vitro immunization.

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IAR42414505	Monoclonal Anti-CMKLR1 antibody produced in rat			Price
IAR4246036	Monoclonal Anti-PAX9 antibody produced in rat			Price

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