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Bioreactor Cultivation of Escherichia Coli for Production of Recombinant Penicillin G Amidase From Alcaligenes Faecalis

Peter M Deak, Sabine Lutz-Wahl, Harald Bothe, Lutz Fischer

Biotechnol Lett. 2003 Mar;25(5):397-400.

PMID: 12882560

Abstract:

The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter. Cells were grown in a synthetic minimal medium in a bioreactor (5 l working vol.), and production of PGA was induced by repeated addition of the inducer rhamnose, that served also as a carbon source. The fermentation yield was about 4500 units PGA activity per liter of culture medium.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP9014066 Penicillin Amidase from Escherichia coli Penicillin Amidase from Escherichia coli 9014-06-6 Price
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