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Biosynthesis of 3-hydroxypropionic Acid From Glycerol in Recombinant Escherichia Coli Expressing Lactobacillus Brevis dhaB and dhaR Gene Clusters and E. Coli K-12 aldH

Biosynthesis of 3-hydroxypropionic Acid From Glycerol in Recombinant Escherichia Coli Expressing Lactobacillus Brevis dhaB and dhaR Gene Clusters and E. Coli K-12 aldH

Suryang Kwak, Yong-Cheol Park, Jin-Ho Seo

Bioresour Technol. 2013 May;135:432-9.

PMID: 23246300

Abstract:

3-Hydroxypropionic acid (3-HP) is a value-added chemical for polymer synthesis. For biosynthesis of 3-HP from glycerol, two dhaB and dhaR clusters encoding glycerol dehydratase and its reactivating factor, respectively, were cloned from Lactobacillus brevis KCTC33069 and expressed in Escherichia coli. Coexpression of dhaB and dhaR allowed the recombinant E. coli to convert glycerol to 3-hydroxypropionaldehyde, an intermediate of 3-HP biosynthesis. To produce 3-HP from glycerol, fed-batch fermentation with a two-step feeding strategy was designed to separate the cell growth from the 3-HP production stages. Finally, E. coli JHS00947 expressing L .brevis dhaB and dhaR, and E. coli aldH produced 14.3g/L 3-HP with 0.26 g/L-h productivity, which were 14.6 and 8.53 times higher than those of the batch culture. In conclusion, overexpression of L. brevis dhaB and dhaR clusters and E. coli aldH, and implementation of the two-step feeding strategy enabled recombinant E. coli to convert glycerol to 3-HP efficiently.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9001609-A	Lactic Dehydrogenase, recombinant from E. coli		9001-60-9	Price

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