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Biosynthesis of Functionally Active Heparin Cofactor II by a Human Hepatoma-Derived Cell Line

Biosynthesis of Functionally Active Heparin Cofactor II by a Human Hepatoma-Derived Cell Line

E A Jaffe, D Armellino, D M Tollefsen

Biochem Biophys Res Commun. 1985 Oct 15;132(1):368-74.

PMID: 2998359

Abstract:

Human plasma heparin cofactor II (HCII) inhibits thrombin by rapidly forming a stable, equimolar complex in the presence of heparin or dermatan sulfate. Cultured human hepatoma-derived cells (PLC/PRF-5) secreted (approximately equal to 200 ng/ml in 3 days) a protein of MW - 72 kD that was immunoisolated and immunoblotted with anti-HCII, co-migrated on SDS-PAGE with human plasma HCII, and formed covalent complexes with thrombin (MW - 101 kD) in the presence but not absence of heparin or dermatan sulfate; these complexes co-migrated with those obtained by incubating thrombin with human plasma under the same conditions. HCII was not detectable (less than 0.13 ng/ml) in post-culture medium from cultured human umbilical vein endothelial cells or human foreskin fibroblasts.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42415748	Heparin Cofactor II from human plasma			Price

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