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Biosynthesis of Leupeptin. IV. Is Protein Turnover in Leupeptin Producer Cells Affected by Leupeptin?

Biosynthesis of Leupeptin. IV. Is Protein Turnover in Leupeptin Producer Cells Affected by Leupeptin?

K Suzukake, M Takada, M Hori, H Umezawa

J Antibiot (Tokyo). 1980 Oct;33(10):1172-6.

PMID: 7451369

Abstract:

There was no significant difference between the rates of protein degradation in cells of a leupeptin-producing strain and that of a leupeptin-nonproducing strain, the latter being derived from the former by mutation. Protein autodigestion in a cell homogenate of the leupeptin producer was sensitive to EDTA and chymotrypsin and less sensitive to leupeptin. On the contrary, protein degradation caused by exogenous trypsin in a similar homogenate was highly sensitive to leupeptin. A labeling experiment with [14C]-arginine of a culture of the leupeptin producer strain revealed that leupeptin was accumulated mostly in the medium and only slightly in the cells; the ratio between the amount in the medium and that in the cells was about 250:1. In contrast, leupeptin acid, the proximal intermediate having no antiplasmin activity, showed a ratio of 5:1.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP103476897	Leupeptin		103476-89-7	Price

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