Home
Resources
Publications
Biosynthesis of Nerol From Glucose in the Metabolic Engineered Escherichia Coli

Biosynthesis of Nerol From Glucose in the Metabolic Engineered Escherichia Coli

Zhen Zong, Qingsong Hua, Xinyu Tong, Dongsheng Li, Chao Wang, Daoyi Guo, Zhijie Liu

Bioresour Technol. 2019 Sep;287:121410.

PMID: 31076292

Abstract:

In this study, nerol was biosynthesized in the metabolic engineered Escherichia coli from glucose for the first time. Firstly, the truncated neryl diphosphate synthase gene tNDPS1 was expressed that catalyzes isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) to form neryl diphosphate (NPP), and then the nerol synthase gene GmNES was co-expressed to synthesize the final product nerol from NPP. The engineered strain LZ001 accumulated 0.053 ± 0.015 mg/L of nerol. Secondly, the IDI1, MVD1, ERG8, ERG12, tHMG1 and ERG13 were co-expressed to increase the supply of IPP and DMAPP. Finally, the heterologous ERG10 gene was overexpressed, and the recombinant strain LZ005 produced 1.564 ± 0.102 mg/L of nerol in shaking-flask culture, which represents a 29.51-fold increase over LZ001 strain. This study shows the novel method for the biosynthesis of nerol and provides new metabolic engineering strategy for the production of terpenoids.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP106252	Nerol		106-25-2	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: