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Biotin-hydrazide Derivatives for the Development of Steroid Enzyme-Linked Immunoassays

C E Gómez-Sánchez, L M León, E P Gómez-Sánchez

J Steroid Biochem Mol Biol. 1992 Nov;43(6):523-7.

PMID: 1419887

Abstract:

We are describing the synthesis and use of biotinamidocaproyl-derivatives of 18-oxocortisol-3-carboxymethoxylamine for the development of enzyme labels using the avidin-peroxidase system for the design of enzyme-linked immunoassays (ELISA). An ELISA for 18-oxocortisol and -hydroxycorticosterone was devised which showed improved sensitivity and specificity in comparison to an RIA using a tritiated tracer. The system is easy to prepare and offers the possibility to design immunoassays when no tritiated tracer is available.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP66640866 (+)-Biotin hydrazide (+)-Biotin hydrazide 66640-86-6 Price
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