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Biotin Uptake: Influx, Efflux and Countertransport in Escherichia Coli K12

Biotin Uptake: Influx, Efflux and Countertransport in Escherichia Coli K12

A Piffeteau, M Gaudry

Biochim Biophys Acta. 1985 Jun 11;816(1):77-82.

PMID: 3890946

Abstract:

Biotin uptake by Escherichia coli K12 has been reinvestigated. The vitamin uptake is an active process depending on energy and inhibited by uncouplers. The kinetic parameters (Km = 0.27 microM, Vmax = 6.8 pmol/min per mg dry cells) are close to those previously determined for a biotin-dependent strain E. coli C162 (Piffeteau, A., Zamboni, M. and Gaudry, M. (1982) Biochim. Biophys. Acta 688, 29-36). By use of biotin p-nitrophenyl ester, an affinity label of the biotin transport system, it was shown, under conditions of steady state, that the efflux of biotin is not energy dependent and is mainly mediated by a diffusion mechanism. Reexamination of the regulation of the biotin transport by biotin, revealed that only 50% of the biotin uptake system is under control by the vitamin.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42415182	β-Galactosidase-biotin labeled from Escherichia coli			Price

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