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Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G Codreanu, Hye-Young H Kim, Ned A Porter, Daniel C Liebler

Methods Mol Biol. 2012;803:77-95.

PMID: 22065220

Abstract:

Formation of covalent protein adducts by lipid electrophiles contributes to diseases and toxicities linked to oxidative stress, but analysis of the adducts presents a challenging analytical problem. We describe selective adduct capture using biotin affinity probes to enrich protein and peptide adducts for analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS). One approach employs biotinamidohexanoic acid hydrazide to covalently label residual carbonyl groups on adducts. The other employs alkynyl analogs of lipid electrophiles, which form adducts that can be postlabeled with azidobiotin tags by Cu(+)-catalyzed cycloaddition (Click chemistry). To enhance the selectivity of adduct capture, we use an azidobiotin reagent with a photocleavable linker, which allows recovery of adducted proteins and peptides under mild conditions. This approach allows both the identification of protein targets of lipid electrophiles and sequence mapping of the adducts.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP109276348	(+)-Biotinamidohexanoic acid hydrazide		109276-34-8	Price

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