Home
Resources
Publications
Biotransformation of Hydroxycoumarin Derivatives by Cultured Suspension Cells of Catharanthus Roseus

Biotransformation of Hydroxycoumarin Derivatives by Cultured Suspension Cells of Catharanthus Roseus

Bailin Xue, Liangbin Zhou, Jinwei Liu, Rongmin Yu

Pharmazie. 2012 May;67(5):467-71.

PMID: 22764584

Abstract:

Using 7-hydroxy-4-methylcoumarin (1), 7-hydroxy-4-phenylcoumarin (2), 5,7-dihydroxy-4-methylcoumarin (3), 7,8-dihydroxycoumarin (4) as substrates, three new compounds, 4-methylcoumarin-7-O-beta-D-xylopyranosyl (1 --> 6) beta-D-glucopyranoside (1b), 4-phenylcoumarin-7-O-beta-D-glucopyranosyl (1 --> 6) beta-D-glucopyranoside (2b), 4-methylcoumarin-5,7-O-beta-D-diglucoside (3b), along with four known compounds (1a, 2a, 3a and 4a) were biosynthesized by the cell suspension cultures of Catharanthus roseus as biocatalyst. Their structures were elucidated by analysis of the physicochemical properties and 1H NMR, 13C NMR, HMBC and ESI-MS spectra. Besides, the results also showed that this novel biocatalyst system of C. roseus cell cultures has a great capacity of regio-selective glycosylation to the hydroxyl group of the exogenous substrates. Co-culture time curves on conversion were also established.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP2107768	5,7-Dihydroxy-4-methylcoumarin		2107-76-8	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: