Home
Resources
Publications
Biphasic Ca2+ Responses in Human Basophils. Evidence That the Initial Transient Elevation Associated With the Mobilization of Intracellular Calcium Is an Insufficient Signal for Degranulation

Biphasic Ca2+ Responses in Human Basophils. Evidence That the Initial Transient Elevation Associated With the Mobilization of Intracellular Calcium Is an Insufficient Signal for Degranulation

D MacGlashan Jr, L M Botana

J Immunol. 1993 Feb 1;150(3):980-91.

PMID: 7678630

Abstract:

Human basophils exhibited biphasic cytosolic Ca2+ responses after stimulation with both the bacterial peptide, FMLP and IgE cross-linking agents such as goat polyclonal anti-IgE PS myeloma (anti-IgE) antibody and Ag. The biphasic response to anti-IgE antibody was not apparent unless supraoptimal concentrations of anti-IgE antibody were used to stimulate the cells. Biphasic response characteristics were also observed in single cells. As in many cell types, the first phase of the Ca2+ response appeared independent of extracellular Ca2+. Challenging basophils with anti-IgE in the presence of EGTA, added simultaneously or 3 to 5 min before the stimulus, had little effect on the first transient Ca2+ elevation while completely eliminating the second phase of the response. We noted that while simultaneous addition of EGTA had little effect on the histamine release after challenge with FMLP, there was no histamine release after the simultaneous addition of anti-IgE and EGTA. An examination of the kinetics of histamine release after anti-IgE also demonstrated that no histamine release occurs during the initial transient response. Since the initial Ca2+ transient was intact after both stimuli in the presence of EGTA, we concluded that the initial transient was not sufficient to induce histamine release. The hypothesis that distinct sources of calcium were responsible for the initial Ca2+ transient that followed challenge with FMLP peptide or anti-IgE antibody was also tested. We found that prior treatment of basophils with thapsigargin inhibited the initial Ca2+ response that followed both FMLP and anti-IgE antibody. Furthermore, stimulation with FMLP, in the presence of EGTA, depleted the calcium source responsible for the anti-IgE response. The experiment conducted in reverse order showed that anti-IgE antibody could discharge the calcium source responsible for the initial FMLP Ca2+ response. These experiments indicated that FMLP and anti-IgE antibody use the same internal source of calcium to produce the initial Ca2+ response. Taken together with previous studies on FMLP, anaphylatoxin split product of the C component, C5; and platelet activating factor-induced release in human basophils, these results suggest that the initial transient elevation, which is relatively independent of extracellular Ca2+, is not sufficient to induce degranulation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4246784	Anti-CACNB4 (Internal) antibody produced in goat			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: