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Blue Dextran: Influence on Chromatographic Profile and Immunoreactivity of Human Follicle-Stimulating Hormone (HFSH)

J Bell, E Rosenkovich, D Rabinowitz

Proc Soc Exp Biol Med. 1975 Jun;149(2):565-9.

PMID: 1080281

Abstract:

When FSH is chromatographed on G-100 Sephadex in the absence of Blue Dextran, it elutes as a single peak in the region of monomeric FSH. The addition of Blue Dextran causes a significant portion of FSH, iodinated or uniodinated, to elute in the region of the void volume. When standard curves for FSH are performed under identical conditions, with tracer 125I-FSH from the void volume, and from the FSH monomer region, maximal immunoreactivity was significantly greater for the Blue Dextran-associated tracer.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP87915386 Blue Dextran Blue Dextran 87915-38-6 Price
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