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Blue Fluorescent Protein From the Calcium-Sensitive Photoprotein Aequorin: Catalytic Properties for the Oxidation of Coelenterazine as an Oxygenase

Blue Fluorescent Protein From the Calcium-Sensitive Photoprotein Aequorin: Catalytic Properties for the Oxidation of Coelenterazine as an Oxygenase

Satoshi Inouye, Satoko Sasaki

FEBS Lett. 2006 Apr 3;580(8):1977-82.

PMID: 16545379

Abstract:

Blue fluorescent protein from the calcium-binding photoprotein aequorin (BFP-aq) is a complex of Ca2+ -bound apoaequorin and coelenteramide, and shows luminescence activity like a luciferase, catalyzing the oxidation of coelenterazine with molecular oxygen. To understand the catalytic properties of BFP-aq, various fluorescent proteins (FP-aq) have been prepared from semi-synthetic aequorin and characterized in comparison with BFP-aq. FP-aq has luciferase activity and could be regenerated into native aequorin by incubation with coelenterazine. The results from substrate specificity studies of FP-aq using various coelenterazine analogues have suggested that the oxidation of coelenterazine by BFP-aq in the luciferase reaction and the regeneration process to aequorin might involve the same catalytic site of BFP-aq.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4246617	Coelenterazine ip			Price

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