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BMS-777607 Promotes Megakaryocytic Differentiation and Induces Polyploidization in the CHRF-288-11 Cells

BMS-777607 Promotes Megakaryocytic Differentiation and Induces Polyploidization in the CHRF-288-11 Cells

Retno Wahyu Nurhayati, Yoshihiro Ojima, Masahito Taya

Hum Cell. 2015 Apr;28(2):65-72.

PMID: 25304900

Abstract:

Introduction of a polyploidy inducer is a promising strategy to achieve a high level of polyploidization during megakaryocytic (MK) differentiation. Here, we report that a multi-kinase inhibitor, BMS-777607, is a potent polyploidy inducer for elevating high ploidy cell formation in the MK-differentiated CHRF-288-11 (CHRF) cells. Our result showed that BMS-777607 strongly inhibited cell division without affecting cell viability when detected at day 1 after treatment. As a consequence, the high ploidy (≥8N) cells were accumulated in culture for 8 days, with an increase from 16.2 to 75.2 % of the total cell population. The elevated polyploidization was accompanied by the increased expression level of MK marker, CD41 (platelet glycoprotein IIb/IIIa, GPIIb/IIIa), suggesting that BMS-777607 promoted both polyploidization and commitment of MK-differentiated CHRF cells. Platelet-like fragments (PFs) were released by mature CHRF cells. Based on a flow cytometry assay, it was found that the PFs produced from BMS-777607-treated cells tended to have larger size and higher expression of GPIIb/IIIa, a receptor for platelet adhesion. Taken together, these results suggested that BMS-777607 promoted MK differentiation of CHRF cells and increased the functional property of platelet-like fragments.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1025720948	BMS-777607		1025720-94-8	Price

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