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Boronic Acid-Modified Magnetic Fe 3 O 4@mTiO 2 Microspheres for Highly Sensitive and Selective Enrichment of N-Glycopeptides in Amniotic Fluid

Boronic Acid-Modified Magnetic Fe 3 O 4@mTiO 2 Microspheres for Highly Sensitive and Selective Enrichment of N-Glycopeptides in Amniotic Fluid

Zhonghua Shi, Liyong Pu, Yueshuai Guo, Ziyi Fu, Wene Zhao, Yunxia Zhu, Jindao Wu, Fuqiang Wang

Sci Rep. 2017 Jul 4;7(1):4603.

PMID: 28676633

Abstract:

Although mesoporous materials and magnetic materials are used to enrich glycopeptides, materials sharing both mesoporous structures and magnetic properties have not been reported for glycopeptide analyses. Here we prepared boronic acid-modified magnetic Fe3O4@mTiO2 microspheres by covalent binding of boronic acid molecules onto the surfaces of silanized Fe3O4@mTiO2 microspheres. The final particles (denoted as B-Fe3O4@mTiO2) showed a typical magnetic hysteresis curve, indicating superparamagnetic behavior; meanwhile, their mesoporous sizes did not change in spite of the reduction in surface area and pore volume. By using these particles together with conventional poly(methyl methacrylate) (PMMA) nanobeads, we then developed a synergistic approach for highly specific and efficient enrichment of N-glycopeptides/glycoproteins. Owing to the introduction of PMMA nanobeads that have strong adsorption towards nonglycopeptides, the number of N-glycopeptides detected and the signal-to-noise ratio in analyzing standard proteins mixture both increased appreciably. The recovery of N-glycopeptides by the synergistic method reached 92.1%, much improved than from B-Fe3O4@mTiO2 alone that was 75.3%. Finally, we tested this approach in the analysis of amniotic fluid, obtaining the maximum number and ratio of N-glycopeptides compared to the use of B-Fe3O4@mTiO2 alone and commercial SiMAG-boronic acid particles. This ensemble provides an interesting and efficient enrichment platform for glycoproteomics research.

Chemicals Related in the Paper:

Catalog Number	Product Name	Price
LS793652	3-methoxy-2-hydroxyphenyl boronic acid	Price
LS793655	2-hydroxy-3-methylphenyl boronic acid	Price
LS793877	(4-phenylaminocarbonylphenyl)boronic acid	Price

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