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Bovine Prothrombin Fragment 1, Segment 1-10. Synthesis and Immunological Investigation

Bovine Prothrombin Fragment 1, Segment 1-10. Synthesis and Immunological Investigation

P B Ten Kortenaar, W W Wilkerson, N T Boggs 3rd, D A Madar, K A Koehler, R G Hiskey

Int J Pept Protein Res. 1980 Nov;16(5):440-9.

PMID: 7216619

Abstract:

The N-terminal decapeptide methyl ester, H-Ala-Asn-Lys-Gly-Phe-Leu-Gla-Gla-Val-Arg-OCH3 (16) of bovine prothrombin fragment 1 has been prepared by standard solution techniques, via a fragment coupling strategy. Hexapeptide Boc-Ala-Asn-Lys epsilon (Boc)-Gly-Phe-Leu-OBzl (9) was obtained by coupling Boc-Ala-Asn-Lys epsilon (Boc)Gly-OH (6) to the trifluoroacetate salt of H-Phe-Leu-OBzl (8). Hydrogenolysis of (9) followed by coupling to HCl. H-Gla gamma (OtBu)2-Gla gamma (OtBu)2-Val-Arg(HCl)-OCH3 (14) gave the fully protected decapeptide (15). Treatment of 15 with 90% trifluoroacetic acid followed by ion exchange chromatography of 15 yielded the methyl ester (16). The decapeptide 16 labeled with 125I using the Bolton-Hunter reagent, did not bind to antibodies specific for the calcium ion-induced conformation of bovine fragment 1.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
ALP141923402	Ser-Phe-Leu-Leu-Arg-Asn-amide trifluoroacetate salt		141923-40-2 (free base)	Price
AP342877558	D-Lys-D-Leu-D-Val-D-Phe-D-Phe-D-Ala trifluoroacetate salt		342877-55-8	Price

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