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Broad-spectrum Monoclonal Antibody and a Sensitive Multi-Residue Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Antibacterial Synergists in Samples of Animal Origin

Broad-spectrum Monoclonal Antibody and a Sensitive Multi-Residue Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Antibacterial Synergists in Samples of Animal Origin

Xiaoya Han, Feng Sheng, Dexin Kong, Yulian Wang, Yuanhu Pan, Mo Chen, Yanfei Tao, Zhenli Liu, Saeed Ahmed, Zonghui Yuan, Dapeng Peng

Food Chem. 2019 May 15;280:20-26.

PMID: 30642487

Abstract:

To monitor the abuse of antibacterial synergists, a hapten, trimethoprim carboxylic derivative (TMPCOOH), was designed by using molecular modelling technology. A broad-spectrum monoclonal antibody (mAb) TMP/2G1 was prepared, for which the IC50 values of trimethoprim, diaveridine, aditoprim, baquiloprim, ormetoprim, and brodimoprim were 0.232, 0.527, 1.479, 4.354, 0.965, and 0.119 µg L-1, respectively. Based on the broad spectrum mAb, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed to determine the residues of antibacterial synergists. The limit of detection regarding the developed ic-ELISA for antibacterial synergists ranged from 0.025 to 1.126 µg L-1 in milk, honey and edible animal tissues. The recoveries ranged from 81.4% to 107.7%, with a coefficient of variation less than 20%. A good correlation (R2 = 0.994) between the ic-ELISA and HPLC-MS/MS showed the reliability of the developed ic-ELISA.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP102280353	Baquiloprim		102280-35-3	Price

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