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C6-Deoxy Coelenterazine Analogues as an Efficient Substrate for Glow Luminescence Reaction of nanoKAZ: The Mutated Catalytic 19 kDa Component of Oplophorus Luciferase

C6-Deoxy Coelenterazine Analogues as an Efficient Substrate for Glow Luminescence Reaction of nanoKAZ: The Mutated Catalytic 19 kDa Component of Oplophorus Luciferase

Satoshi Inouye, Jun-ichi Sato, Yuiko Sahara-Miura, Suguru Yoshida, Hajime Kurakata, Takamitsu Hosoya

Biochem Biophys Res Commun. 2013 Jul 19;437(1):23-8.

PMID: 23792095

Abstract:

The codon-optimized gene for the mutated 19 kDa protein (nanoKAZ), which is the catalytic component of Oplophorus luciferase, was expressed in Escherichia coli cells and the recombinant protein was highly purified. The secretory expression of nanoKAZ from CHO-K1 cells was performed by fusing the secretory signal peptide sequence of Gaussia luciferase to the amino-terminus of nanoKAZ. The substrate specificity for the purified nanoKAZ and the nanoKAZ secreted into the cultured medium was determined, indicating that bis-coelenterazine (bis-CTZ) and newly synthesized 6h-f-coelenterazine (6h-f-CTZ) are an efficient substrate for the glow luminescence reaction of nanoKAZ.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4246617	Coelenterazine ip			Price

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