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C9ORF72-ALS/FTD-associated poly(GR) Binds Atp5a1 and Compromises Mitochondrial Function in Vivo

C9ORF72-ALS/FTD-associated poly(GR) Binds Atp5a1 and Compromises Mitochondrial Function in Vivo

So Yoen Choi, Rodrigo Lopez-Gonzalez, Gopinath Krishnan, Hannah L Phillips, Alissa Nana Li, William W Seeley, Wei-Dong Yao, Sandra Almeida, Fen-Biao Gao

Nat Neurosci. 2019 Jun;22(6):851-862.

PMID: 31086314

Abstract:

The GGGGCC repeat expansion in C9ORF72 is the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). However, it is not known which dysregulated molecular pathways are primarily responsible for disease initiation or progression. We established an inducible mouse model of poly(GR) toxicity in which (GR)80 gradually accumulates in cortical excitatory neurons. Low-level poly(GR) expression induced FTD/ALS-associated synaptic dysfunction and behavioral abnormalities, as well as age-dependent neuronal cell loss, microgliosis and DNA damage, probably caused in part by early defects in mitochondrial function. Poly(GR) bound preferentially to the mitochondrial complex V component ATP5A1 and enhanced its ubiquitination and degradation, consistent with reduced ATP5A1 protein level in both (GR)80 mouse neurons and patient brains. Moreover, inducing ectopic Atp5a1 expression in poly(GR)-expressing neurons or reducing poly(GR) level in adult mice after disease onset rescued poly(GR)-induced neurotoxicity. Thus, poly(GR)-induced mitochondrial defects are a major driver of disease initiation in C9ORF72-related ALS/FTD.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP124555186	GR 79236X		124555-18-6	Price

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