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Caerulomycin A Suppresses Immunity by Inhibiting T Cell Activity

Caerulomycin A Suppresses Immunity by Inhibiting T Cell Activity

Arvind K Singla, Rama Krishna Gurram, Arun Chauhan, Neeraj Khatri, Rakesh M Vohra, Ravinder S Jolly, Javed N Agrewala

PLoS One. 2014 Oct 6;9(10):e107051.

PMID: 25286329

Abstract:

Background:
Caerulomycin A (CaeA) is a known antifungal and antibiotic agent. Further, CaeA is reported to induce the expansion of regulatory T cell and prolongs the survival of skin allografts in mouse model of transplantation. In the current study, CaeA was purified and characterized from a novel species of actinomycetes, Actinoalloteichus spitiensis. The CaeA was identified for its novel immunosuppressive property by inhibiting in vitro and in vivo function of T cells.
Methods:
Isolation, purification and characterization of CaeA were performed using High Performance Flash Chromatography (HPFC), NMR and mass spectrometry techniques. In vitro and in vivo T cell studies were conducted in mice using flowcytometry, ELISA and thymidine-[methyl-(3)H] incorporation.
Results:
CaeA significantly suppressed T cell activation and IFN-γ secretion. Further, it inhibited the T cells function at G1 phase of cell cycle. No apoptosis was noticed by CaeA at a concentration responsible for inducing T cell retardation. Furthermore, the change in the function of B cells but not macrophages was observed. The CaeA as well exhibited substantial inhibitory activity in vivo.
Conclusion:
This study describes for the first time novel in vitro and in vivo immunosuppressive function of CaeA on T cells and B cells. CaeA has enough potential to act as a future immunosuppressive drug.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP21802379	Caerulomycin A		21802-37-9	Price

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