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Calcium Stoichiometry Determination for Calcium Binding Proteins by Electrospray Ionization Mass Spectrometry

Calcium Stoichiometry Determination for Calcium Binding Proteins by Electrospray Ionization Mass Spectrometry

P Hu, Q Z Ye, J A Loo

Anal Chem. 1994 Dec 1;66(23):4190-4.

PMID: 7847625

Abstract:

Electrospray ionization mass spectrometry was used for the determination of calcium-binding stoichiometry for calcium-binding proteins. Bovine calmodulin, bovine alpha-lactalbumin, and rabbit parvalbumin were found to bind specifically to 4, 1, and 2 Ca(2+) ions, respectively, in agreement with previously reported results obtained by using other physical methods. This mass spectrometry method could also be applied to proteins that bind more than one type of metal ion. The Zn(2+)- and Ca(2+)-binding stoichiometries for human stromelysin catalytic domain were determined to be 3 and 2, respectively.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR424828	Calmodulin bovine			Price

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