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Calmodulin-dependent Cyclic Nucleotide Phosphodiesterase From Bovine Eye: High Calmodulin Affinity Isozyme Immunologically Related to the Brain 60-kDa Isozyme

Calmodulin-dependent Cyclic Nucleotide Phosphodiesterase From Bovine Eye: High Calmodulin Affinity Isozyme Immunologically Related to the Brain 60-kDa Isozyme

R K Sharma, Y Tan, R V Raju

Arch Biochem Biophys. 1997 Mar 1;339(1):40-6.

PMID: 9056231

Abstract:

Calmodulin-dependent cyclic nucleotide phosphodiesterase was identified in and purified to apparent homogeneity from the total calmodulin-binding protein fraction of bovine eye in a single step by immunoaffinity chromatography. The bovine eye calmodulin-dependent cyclic nucleotide phosphodiesterase is immunologically similar to the bovine brain 60-kDa isozyme. The purified enzyme had higher affinity for calmodulin than the 60-kDa phosphodiesterase isozyme from bovine brain, but similar affinity to that of the heart isozyme. When the Ca(2+)-dependence of the eye enzyme was compared to cardiac calmodulin-dependent cyclic nucleotide phosphodiesterase at an identical concentration of calmodulin, the bovine eye calmodulin-dependent cyclic nucleotide phosphodiesterase was activated at the same Ca2+ concentration as the bovine heart calmodulin-dependent cyclic nucleotide phosphodiesterase isozyme.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4241092	Calmodulin from bovine brain			Price

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