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Calycopterin Promotes Survival and Outgrowth of Neuron-Like PC12 Cells by Attenuation of Oxidative- And ER-stress-induced Apoptosis Along With Inflammatory Response

Calycopterin Promotes Survival and Outgrowth of Neuron-Like PC12 Cells by Attenuation of Oxidative- And ER-stress-induced Apoptosis Along With Inflammatory Response

Mahdi Moridi Farimani, Nazanin Namazi Sarvestani, Niloufar Ansari, Fariba Khodagholi

Chem Res Toxicol. 2011 Dec 19;24(12):2280-92.

PMID: 22081883

Abstract:

There is mounting evidence implicating the role of oxidative stress induced by reactive oxygen species (ROS) in neurodegenerative disease, including Alzheimer's disease. Herein we investigated the neuroprotective potential of a natural flavonoid, calycopterin, against H(2)O(2)-induced cell death in differentiated PC12 cells. We pretreated PC12 cells with 25, 50, and 100 μM calycopterin followed by the addition of H(2)O(2) as an oxidative stress agent. We measured cell viability by the MTT test and found that 50 μM is the best protective concentration of calycopterin. Moreover, we measured six different parameters of neurite outgrowth. Interestingly, we found that calycopterin not only protects PC12 cells against H(2)O(2)-induced apoptosis but also defends against the destructive effect of oxidative stress on the criteria of neural differentiation. Calycopterin decreased ER stress-associated proteins including calpain and caspase-12, and suppressed ERK, JNK, and p38 MAPK phosphorylation. Moreover, calycopterin inhibited H(2)O(2)-induced nuclear translocation of nuclear factor-κB, a known regulator of a host of genes involved in specific stress and inflammatory responses. This observation was perfectly in agreement with the decrease of COX-2 and TNF-α levels. Calycopterin reduced intracellular ROS levels and increased catalase activity. The protective effect of this compound could represent a promising approach for the treatment of neurodegenerative diseases.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP481527	Calycopterin		481-52-7	Price

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