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Candida Sp. 99-125 Lipase-Catalyzed Synthesis of Ergosterol Linolenate and Its Characterization

Candida Sp. 99-125 Lipase-Catalyzed Synthesis of Ergosterol Linolenate and Its Characterization

Wen-Sen He, Lingling Li, Jia Zhao, Hanshan Xu, Jiaxin Rui, Dandan Cui, He Li, Huijuan Zhang, Xinqi Liu

Food Chem. 2019 May 15;280:286-293.

PMID: 30642499

Abstract:

As a major sterol in edible mushroom, ergosterol has gained much attention owing to its potential bioactivities. However, ergosterol has a high melting point, poor oil solubility and stability, which restrict its scope of application. In this study, an ergosterol ester of α-linolenic acid was successfully and efficiently prepared using Candida sp. 99-125 lipase as a biocatalyst. The desired product was confirmed to be ergosterol linolenate using MS, FT-IR, and NMR analyses. Using Candida sp. 99-125 lipase, the product conversion exceeded 92% in 12 h under the following optimized parameters: 75 mmol/L ergosterol, 40 g/L lipase, 1:1.25 ergosterol-to-α-linolenic acid molar ratio, and 45 °C. The results confirmed that Candida sp. 99-125 lipase has good reusability and stability and is also relatively low cost, suggesting its great potential for large-scale production of ergosterol ester. Most importantly, the physiochemical properties (oil solubility and melting point) of ergosterol significantly improved after esterification with α-linolenic acid, thus facilitating its application in oil-based systems.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR424808	Lipase from Candida sp.			Price

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