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Carbonyl Cyanide-M-Chlorophenyl Hydrazone-Resistant Escherichia Coli Mutant That Exhibits a Temperature-Sensitive Unc Phenotype

Carbonyl Cyanide-M-Chlorophenyl Hydrazone-Resistant Escherichia Coli Mutant That Exhibits a Temperature-Sensitive Unc Phenotype

M Ito, Y Ohnishi, S Itoh, M Nishimura

J Bacteriol. 1983 Jan;153(1):310-5.

PMID: 6217194

Abstract:

Two spontaneous Escherichia coli mutant strains which are resistant to an oxidative phosphorylation uncoupler, carbonyl cyanide-m-chlorophenyl hydrazone, were isolated. Strain CM22 (ccr-2) was resistant to another uncoupler, pentachlorophenol, and to the inhibitors of proton-translocating ATPase, namely tributyltin and sodium azide. Carbonyl cyanide-m-chlorophenyl hydrazone or pentachlorophenol administered to cell suspensions of strain CM22 did not cause a pH change induced by H+ influx, and a similar result was obtained with everted particles. The respiratory rate of strain CM22 with succinate was twice that of wild-type strain KH434. When carbonyl cyanide-m-chlorophenyl hydrazone was administered, a stimulation of O2 uptake was observed in wild-type strain KH434 but not in the mutant strain CM22. Strain CM22 did not grow on succinate at 42 degrees C. Isolation of a true revertant at a frequency of 10(-8) demonstrated that the pleiotropic phenotype was induced by a single mutation. P1 transduction indicated that the mutant allele, ccr-2, was cotransduced with the ilv genes at a frequency of about 55%.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP2179922	Tributyltin cyanide		2179-92-2	Price

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