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Cardiomyocyte Enrichment From Human Embryonic Stem Cell Cultures by Selection of ALCAM Surface Expression

Cardiomyocyte Enrichment From Human Embryonic Stem Cell Cultures by Selection of ALCAM Surface Expression

William Rust, Thavamalar Balakrishnan, Robert Zweigerdt

Regen Med. 2009 Mar;4(2):225-37.

PMID: 19317642

Abstract:

Aims:
The production of a homogenous population of human cardiomyocytes that can be expanded in vitro may facilitate development of replacement tissue lost as a result of cardiac disease and injury.
Materials and methods:
We evaluated the utility of activated leukocyte cell-adhesion molecule, CD166 (ALCAM) expression as a marker for isolating cardiomyocytes from differentiating cultures of human embryonic stem cells (hESCs). Using RT-qPCR, immunohistochemistry and DNA methylation studies, we evaluated the developmental age of hESC-derived cardiomyocytes.
Results and conclusions:
We demonstrate that cardiomyocytes derived from hESC cultures express ALCAM and that this surface antigen can be used to select a population of differentiated cells that are enriched for cardiomyocytes. Expression of contractile proteins and ion channels, and DNA methylation patterns, suggest that ALCAM-enriched cardiomyocytes have an embryonic phenotype. Selected cardiomyocyte populations survive sorting, adhere to collagen-coated tissue culture plastic and proliferate in short-term culture. Long-term in vitro survival of cardiomyocytes was achieved by culturing cells in 3D aggregates.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413199	CD166/ALCAM human			Price

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