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Cathepsin B-deficient Mice as Source of Monoclonal Anti-Cathepsin B Antibodies

Cathepsin B-deficient Mice as Source of Monoclonal Anti-Cathepsin B Antibodies

Ekkehard Weber, Elena Barbulescu, Rita Medek, Thomas Reinheckel, Mansoureh Sameni, Arulselvi Anbalagan, Kamiar Moin, Bonnie F Sloane

Biol Chem. 2015 Mar;396(3):277-81.

PMID: 25205719

Abstract:

Cathepsin B has been demonstrated to be involved in several proteolytic processes that support tumor progression and metastasis and neurodegeneration. To further clarify its role, defined monoclonal antibodies are needed. As the primary structure of human cathepsin B is almost identical to that of the mouse, cathepsin B-deficient mice were used in a novel approach for generating such antibodies, providing the chance of an increased immune response to the antigen, human cathepsin B. Thirty clones were found to produce cathepsin B-specific antibodies. Seven of these antibodies were used to detect cathepsin B in MCF10-DCIS human breast cancer cells by immunocytochemistry and immunoblotting. Five different binding sites were identified by epitope mapping giving the opportunity to combine these antibodies in oligoclonal antibody mixtures for an improved detection of cathepsin B.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42412601	Anti-Cathepsin B antibody, Mouse monoclonal			Price

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