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Cell Uptake and Validation of Novel PECs for Biomedical Applications

Cell Uptake and Validation of Novel PECs for Biomedical Applications

Ilaria E Palamà, Mariarosaria Musarò, Addolorata M L Coluccia, Stefania D'Amone, Giuseppe Gigli

J Drug Deliv. 2011;2011:203676.

PMID: 21876815

Abstract:

This pilot study provides the proof of principle for biomedical application of novel polyelectrolyte complexes (PECs) obtained via electrostatic interactions between dextran sulphate (DXS) and poly(allylamine hydrochloride) (PAH). Scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed that DXS/PAH polyelectrolyte complexes were Monodispersed with regular rounded-shape features and average diameters of 250 nm at 2 : 1 weight ratios of DXS/PAH. Fluorescently labelled DXS and fluorescein-isothiocyanate- (FITC-)conjugate DXS were used to follow cell uptake efficiency of PECs and biodegradability of their enzymatically degradable DXS-layers by using confocal laser scanning microscopy (CLSM). Moreover, quantitative MTT and Trypan Blue assays were employed to validate PECs as feasible and safe nanoscaled carriers at single-cell level without adverse effects on metabolism and viability.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS775181	Poly(fluorescein isothiocyanate allylamine hydrochloride)			Price

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