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Cell Viability Measurement Using 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein Acetoxymethyl Ester and a Cantilever Sensor

Cell Viability Measurement Using 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein Acetoxymethyl Ester and a Cantilever Sensor

Sen Xu, Raj Mutharasan

Anal Chem. 2011 Feb 15;83(4):1480-3.

PMID: 21244099

Abstract:

Detection of viable pathogenic bacteria has widespread application in food safety and human health. Antibody-based methods require a growth step which limits time-to-results performance. In this study, we use a mass-change sensitive cantilever biosensor and a probe, 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM), that accumulates only in live cells inducing a mass-change response to determine the cell viability in a short time. A poly-L-lysine coated sensor immobilized with live Escherichia coli JM101 (a surrogate for a pathogenic target) at various concentrations was exposed to BCECF-AM in a flow arrangement. A larger resonant frequency decrease in response to 100 μL of 60 μM BCECF-AM was observed when the sensor surface cell concentration was increased from 1 090 ± 580 to 3 960 ± 370 cells/mm(2) (n = 5). A log-linear relationship between the sensor surface cell concentration and frequency response was obtained in the range of 1 000-4 000 cells/mm(2) and as low as ∼2 000 viable E. coli cells were rapidly detected (<1 h).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP85138494	2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein		85138-49-4	Price

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